Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: Cell viability ( A ) and cell proliferation ( B ) induced by nicotine in HBEpC and/or si-mRNA-α7-HBEpC viability. ( A ): For cell viability, 7500 cells/cm 2 are plated in T25 flask (total cell number 187,000) and treated with nicotine 1 × 10 −7 M, after 1 h cells are washed three times in PBS Ca 2+ and Mg 2+ free and then incubated in drug-free medium for additionally 48 h. Then, cells (detached or floating) are counted after staining with trypan blue dye. ( B ): For cell proliferation 7500 cells/cm 2 are plated in T25 flask and treated with nicotine every 48 h. Cells are detached and viable cells are counted every 12 h. Experiments are performed at least two times in triplicate. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Incubation, Staining, Comparison

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: Expression of Ki67 induced by nicotine in HBEpC and/or si-mRNA-α7-HBEpC. ( A ): ELISA experiments; ( B ): regression equation linearity, performed with Prism; ( C ): Western blotting, ( D ): densitometric analysis. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate. In the , raw data of Western blotting are reported.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Comparison

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: SA-β-Gal in HBEpC exposed continuously to nicotine. Experiments are performed at least two times in triplicate.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques:

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: Evaluation of intracellular Ca 2+ after exposure to nicotine for 48 h in HBEpC and/or si-mRNA-α7-HBEpC. ( A ): ELISA experiments; ( B ): regression equation linearity, performed with Prism. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Enzyme-linked Immunosorbent Assay, Comparison

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: Evaluation of intracellular ATP after exposure to nicotine for 48 h in HBEpC. ( A ): ELISA experiments; ( B ): regression equation linearity, performed with Prism. Experiments are performed at least two times in triplicate.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: Expression of EGF and p-EGFR after exposure to nicotine for 48 h in HBEpC. ( A ): ELISA experiments for EGFR; ( B ): regression equation linearity for EGFR, performed with Prism; ( C ): ELISA experiments for p-EGFR; ( D ): regression equation linearity for p-EGFR, performed with Prism. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Comparison

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: Induction of p53 and phospho-p53 induced by nicotine in HBEpC. ( A ): ELISA assay. ( B ): Western blotting experiments, ( C ): densometric analysis. Experiments are performed at least two times in triplicate. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. In the , raw data of Western blotting are reported.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Comparison

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: Induction of phospho-p38 and p38 by nicotine in HBEpC. ( A ): ELISA experiments; ( B ): regression equation linearity for phosphor-p38; ( C ): regression equation linearity for phosphor-p38performed with Prism. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Enzyme-linked Immunosorbent Assay, Comparison

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: EMT induced by nicotine in HBEpC. ( A ): Western blotting; ( B ): densitometric analysis. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate. In the , raw data of Western blotting are reported.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Western Blot, Comparison

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: Cell migration to nicotine for 48 h in HBEpC and/or si-mRNA-α7-HBEpC. HeLa cells are positive control. ( A ) HeLa positive control. ( B ): Cell migration h in HBEpC and/or si-mRNA-α7-HBEpC. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Migration, Positive Control, Comparison

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: Induction of VEGF by nicotine in HBEpC and/or si-mRNA-α7-HBEpC. ( A ): ELISA experiments; ( B ) regression equation linearity for VEGFR. Statistical significance is analyzed with one-way AOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Enzyme-linked Immunosorbent Assay, Comparison

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: Anchorage-independent growth induced by nicotine in HBEpC. HeLa cells are positive control and NIH3T3 the negative. ( A ): Representative picture of HBEpC cloned on soft agar. ( B ): Cloned cells. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Positive Control, Clone Assay, Comparison

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: Effects induced by nicotine on different pathways in human airway epithelial cells (results obtained in this work and in literature) and comparison with effects caused by SARS-CoV-2, SARS-CoV, MERS-CoV and by non-tumorigenic virus infection on the same pathways.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Comparison, Virus, Infection, Expressing, Concentration Assay, In Vitro, Activation Assay, Activity Assay, Knockdown, Control, Migration

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Luteolin Inhibits the Biofilm Formation and Cytotoxicity of Methicillin-Resistant Staphylococcus aureus via Decreasing Bacterial Toxin Synthesis

doi: 10.1155/2022/4476339

Figure Lengend Snippet: Luteolin decreased the cytotoxicity of MRSA, and downregulated the expression of the hemolysin and hlaA genes in MRSA (a) Human bronchial epithelial cells were infected with luteolin-treated MRSA N315, and then the viability of human bronchial epithelial cells was measured using the CCK-8 assay. (b) Expressions of α -hemolysin and δ -hemolysin in luteolin-treated MRSA N315 were evaluated by western blot. (c) Level of hlaA in luteolin-treated MRSA N315 was detected using RT-PCR, and 16S was used as an endogenous control. ∗∗∗ P < 0.001, vs. Blank; ## P < 0.01, ### P < 0.001 vs. Control. (MRSA: methicillin-resistant Staphylococcus aureus , CCK-8: Cell Counting Kit-8, 16S: 16S rRNA, a -hemolysin: alpha hemolysin, d -hemolysin: delta hemolysin, RT-PCR: Real-Time PCR).

Article Snippet: Human bronchial epithelial cells (HBEs; CL-0346) were purchased from Procell (Wuhan, China) and grown in the specific cell medium (CM-0346, Procell, Wuhan, China).

Techniques: Expressing, Infection, CCK-8 Assay, Western Blot, Reverse Transcription Polymerase Chain Reaction, Control, Cell Counting, Real-time Polymerase Chain Reaction

Journal: Chemical Research in Toxicology

Article Title: Differential Activation of TRPA1 by Diesel Exhaust Particles: Relationships between Chemical Composition, Potency, and Lung Toxicity

doi: 10.1021/acs.chemrestox.8b00375

Figure Lengend Snippet: Representative photomicrographs of H&E (panels A, C, and E) and PAS (panels B, D, and F) lung tissue from mice collected after treatment with “black smoker” (panels A and B), regenerated filter (panels C and D), or NIST SRM 2975 DEP (panels E and F). The images were captured at 40× using an EVOS FL auto imaging system. (G, H) Relative expression of mRNA for mucin 4 and 5B (MUC4 and MUC5B) by primary human lobar bronchial epithelial cells treated with regenerated filter DEP for 24 h in the presence of absence of the TRPA1 antagonists HC-030031 (20 μM) or A967079 (20 μM). Double asterisks indicate significant inhibition relative to DEP induced mucin mRNA ( p < 0.01) using the one-way ANOVA with Bonferroni’s multiple comparison test.

Article Snippet: Human lobar bronchial epithelial cells (Lobar; donor ID: 01344) were purchased from Lifeline Cell Technology (Frederick, MD).

Techniques: Imaging, Expressing, Inhibition, Comparison